FORMING OF DIASTEREOISOMER WITH L-ISOLEUCIN TO SEPARATE OFLOXACIN ENANTIOMER USING PREPARATIVE LIQUID CHROMATOGRAPHY
ABSTRAK: Ofloxacin is an
antibiotic with a broad range of uses in human and veterinary medicine to
prevent or treat bacterial infections which has an enantiomeric mixture
structure of (-)-ofloxacin and (+)-ofloxacin. Enantiomers of drugs have
different pharmacology and toxicology effects that need to be separated before
it used to get the molecule target. Separation of enantiomers using chiral
column is not quite economical separation, but the separation of enantiomers
using a chiral column by converting the enantiomeric mixture into a
diastereoisomeric form is the right solution. This study aims to separate
mixture of ofloxacin enantiomers by forming diastereoisomeric with L-isoleusine
and copper (II) into compound (-)-ofloxacin and (+)-ofloxacin and also to identify
the optimum condition of separation using HPLC with preparative liquid
chromatography column. Samples injected into the column and eluted by the
mobile phase (consisting a mixture of water-methanol (88:12) and solution of
L-isoleusine 4 mM and copper (II) sulfate 9 mM). Then the fractions were
compared with the standard and the purity was tested by HPLC. This research
resulted 47 fractions which four of early fractions have correspond with the
standard of (-)-ofloxacin and 43 other factions do not. We obtained the
resolution of this separtion about 0.98. The small resolution caused by complex
formation is not perfect with the concentration of L-isoleucine and copper
(II).
Penulis: Diana Hendrati,
Husein H. Bahti, Uji Pratomo, Zenith Putri Dewianti
Kode Jurnal: jpkimiadd150602
