HPLC FINGERPRINT ANALYSIS COMBINED WITH CHEMOMETRICS FOR AUTHENTICATION OF KAEMPFERIA GALANGA FROM RELATED SPECIES
ABSTRACT: Fingerprint analysis
using high performance liquid chromatography (HPLC) has been developed for
authentication of Kaempferia galanga from related species, such as Kaempferia
pandurata and K. rotunda. By comparing the fingerprint chromatograms of K.
galanga, K. pandurata and K. rotunda, we could identify K. galanga samples and
detect adulteration of K. galanga from K. pandurata and K. rotunda by using
their marker peaks. We also combined HPLC fingerprint with chemometrics for
discrimination the three species and also for authentication of K. galanga. All
the three species and K. galanga adulterated with K. pandurata and K. rotunda
were discriminated successfully by using principal component analysis (PCA) and
discriminant analysis (DA). This result indicates that HPLC fingerprint
analysis in combination with PCA (PC1 = 30.06% and PC2 = 34.74%) and DA (DF1 =
94.59% and DF2 = 3.32%) could be used for authentication of K. galanga samples
from the related species.
Author: Cahya Septyanti,
Irmanida Batubara, Mohamad Rafi
Journal Code: jpkimiagg160096
