Induksi Tunas dari Eksplan Kotiledon dan Epikotil In Vitro Jeruk Siam (Citrus nobilis Lour.) Asal Kampar pada Media MS
Abstract: Citrus nobilis Lour.
(Siam Orange) from Kampar is one of the main fruits in Riau Province that is
highlyconsumed by many people, but nowadays its productivity is lower due to
pests and diseases. In order tomaintain the availibility of citrus from Kampar
and to conserve this plant, an effort that can be done is byusing in vitro
culture with the use of a combination of growth regulator; cytokine and auxin
usingcotyledons and epicotyl explant. This study aimed to determine the best
explant source and to know theeffect of the concentration combination of BAP,
kinetin, and NAA that optimal in inducing plant shoot ofSiam orange. This study
was conducted at Laboratory of Integrated Biology, Department of
Biology,Faculty of Mathematics and Natural Sciences using a completely
randomized design (CRD) with variousconcentrations of treatments, and observed
for 42 days. The results showed that the percentage of lifeexplants, swelling
cotyledon, and epicotyl explants was 100%, the percentage of best shoot
formation was60% on the epicotyls explant with 1.0 mg/l BAP + 0.5 mg/l kinetin
+ 0.5 mg/l NAA. The percentage ofcallus formation only found in epicotyls
explants with the highest percentage (100%) at 2.0 mg/l BAP +0.5 mg/l kinetin
+1.0 mg/l NAA.
Penulis: SRI CAHYATI, MAYTA
NOVALIZA ISDA, WAHYU LESTARI
Kode Jurnal: jpbiologidd160587