ISOLASI DAN KARAKTERISASI SENYAWA METABOLIT SEKUNDER FRAKSI ETIL ASETAT DARI KULIT BATANG TUMBUHAN Bauhinia purpurea L
Abstract: Isolation and
characterization secondary metabolite of ethyl acetate fraction from Bauhinia
purpurea L stem bark has been conducted. Bauhinia purpurea L belonging to
biggest family of flowering plant in Asia. This plant was choosed as study
sample evidence remedy effect for some disease like ulcer, cough, tumor,
puffing, and snakebite. Isolation conducted by extraction and chromatograph
method. Extraction by maceration technique used non polar and semipolar solvent
alternally, n-hexane, dichloromethane, and ethyl acetate. The extract separated
by liquid vacuum, coloum, and flash chromatograph until obtained pure compound.
The melting point of compound was determined by John-Fisher apparatus.The
compound structure was determined by UV-Vis, IR, 13C-NMR spectroscopic.The
compound spectroscopy data was compared with standard compound data.Extraction
resulted n-hexane fraction, dichloromethane fraction, and ethyl acetate
fraction. Separation of ethyl acetate fraction resulted white crystalline
powder and has melting point 220-2210C. Evidence on spectroscopy data were
estimated the compound was a stigmasterol glycoside.
Penulis: Ardiansyah, Herdini.,
Abdullah
Kode Jurnal: jpkimiadd150611
