Kinetika dan Karakterisasi Amiloglukosidase Isolat Aaspergillus niger Dari Limbah Ampas Sagu

Abstract:  Study of characterization and kinetic amyloglucosidase from Aspergillusniger isolate using sago pith waste has been done. The aims of this research wereto know the optimumsubstrate concentration, metalions effect, and value of KM and vmaks of amyoglucosidase. Theresult of researches were obtained the optimum activity of amyoglucosidaseat 2% (w/v) ofstarch concentration. The activator of amyoglucosidase were Zn2+, Ba2+, Fe3+, and Co2+,while the inhibitor were Sn2+, Ca2+, Cu2+, Cd2+, Mn2+, Hg2+, and Ni2+. The ions of Hg2+ andNi2+were founded stopped of the enzyme activity. The variables of kinetic of amyloglucosidaewere obtained KM and vmaks value were. 4,40 % , 0,49 mU, respectively.
Kata Kunci: Amiloglukosidase, Ampas Sagu, Aspergillus niger, Kinetika dan Karakterisasi
Penulis: Amiruddin
Kode jurnal: jpkimiadd110190

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Jp Kimia dd 2011