VALIDASI METODE PENENTUAN SAKARIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI
Abstract: This research was
aimed to validation analytical method of Saccarine and to determine saccarine
consentration in soft drink High Performance Liquid Chromatography. Sodium
Saccarine salts are used as standard.
Sodium Saccarine salts dissolve
in mobile phase methanol : fosfat buffer (60:40). Concentration of standard
saccarine solution made in 20, 40, 60, 80, and 100 ppm. HPLC Agilent Technology
1260 Infinitely Series, Poroshell/C18 column- 50 mm lenght- id 4.6 mm - particles size 2.7
µm, UV-detector in λ 265 nm, injection volume 20 µL , and flow rate 1
mL/minutes were used in this research. Method validation parameters were
determined by linierity, precision, accuracy, LOD, LOQ, and selectivity. The
results show that lineariy with R = 0.998702, precision with RSD = 0.192%,
accuracy with % recovery = 100.26%, LOD = 0.172 ppm, LOQ = 0.573, and
selectivity with Rs = 0.2. Testing for the ‘Sprite Zero’ soft drink show that
117.6 mg/kg saccarine (under the maximum content in carbonated/noncarbonated
soft drink). Conclution from this research is,
HPLC method can use in analysis saccarine content in soft drink
Penulis: Agus Kembaren,
Tiasina Harahap
Kode Jurnal: jpkimiadd140874
