A NOVEL SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF HISTAMINE BASED ON ITS COMPLEX REACTION WITH NI(II) AND ALIZARIN RED S
ABSTRACT: The development of
analytical methods of histamine using Ni (II) and alizarin red S reagents by
UV-Vis spectrophotometry has been done. The objective of this research is to
determine the ability of Ni(II) and alizarin red S to form color complex
compound with histamine and it will be used to detect the presence of histamine
qualitatively and quantitatively as well. Absorbance was measured at a maximum
wavelength of 604 nm. In this method, it has been carried out optimization of
analytical parameters such as the concentration of Ni(II), the concentration of
alizarin red S, pH, and respond time. Analytical parameter optimization showed
concentration of Ni(II) is 20 ppm, alizarin red S 75 ppm, pH 8, and a respond
time of 15 minutes. Method validation indicated that the coefficient of
variation, detection limit, and the limit of quantitation are 0.245%; 9.49 ppm;
and 31.62 ppm respectively with a sensitivity of 0.0063/ppm and linearity of
0.99. Accuracy to histamine with a concentration of 50, 75, and 125 ppm are
105.87%, 101.06%, 97.21%, respectively.
Penulis: Miftakhul Jannatin,
Ganden Supriyanto, Pratiwi Pudjiastuti
Journal Code: jpkimiagg170017
