AKTIVITAS ANTIBAKTERI EKSTRAK BIJI KAPULAGA (Amomum compactum Sol. Ex Maton)
Abstract: Testing of
antibacterial activity against ethyl acetate extract local cardamom seeds
(Amomum compactum Sol. Ex Maton) has been performed. Extraction was carried out
using the soxhlet method with methanol solvent and liquid-liquid partitioned
with n-hexane, ethyl acetate and n-butanol solvent, antibacterial activity test
was performed using the disc diffusion method, fractionation using column
chromatography and characterization of active fractions using chromatography
GCMS, UV-vis and FTIR spectroscopy. The test results showed that the
antibacterial activity of ethyl acetate extract had the highest antibacterial
activity against S. aureus and E. coli with inhibition zone diameter of
respectively 15.15 ± 1.34 and 13.50 ± 0.70 mm at a concentration of 3200 mg/mL.
Results of fractionation of the ethyl acetate fraction using column
chromatography with a mobile phase of ethyl acetate: n-hexane (3: 2) yielded
three fractions, namely F1 (14.6 mg), F2 (8.1 mg) and F3 (4.6 mg). Fraction 2
had the highest antibacterial activity against S. aureus with inhibition zone
diameter of 12.34 ± 0.07 mm at a concentration of 800 ug/mL. The results of
the characterization of the fraction 2 obtained using GCMS analysis of three
antibacterial compounds suspected of 2.9-dihydroxy-1,8-cineol;
2,4-dihydroxy-1,8-cineol and 2,2-methylene bis [6- (1,1-dimethylethyl)
-4-ethyl] phenol. The results of the F2 fraction characterization using UV-Vis
spectroscopy showed the presence of group C = C conjugated chromophore at λmax
223 nm and are based on analysis using FTIR there -OH alcohol functional group
(3372 cm-1), aliphatic -CH (2926 and 2854 cm-1) , C = C (1695 cm-1), aliphatic
CH2 (1402 cm-1), CH3 aliphatic (1384 cm-1), and C-O (1203; 1126; 1091 and 1043
cm-1).
Penulis: Dede Sukandar, Sandra
Hermanto, Eka Rizki Amelia, Muhamad Zaenudin
Kode Jurnal: jpkimiadd150646
