Biodegradasi Petroleum dan Hidrokarbon Eikosana oleh Isolat Bakteri Pseudomonas aeruginosa
ABSTRACT: Biodegradation of
petroleum and hydrocarbon eicosane by Pseudomonas aeruginosa isolate.
Hydrocarbon are important environmental contaminants in soil and water. These
compounds have a potential risk to human health, as many of them are
carsinogenic and toxic to marine organisms such as diatome, gasthrophode,
mussel, and fish. The purpose of this research was to know the ability of
Pseudomonas aeruginosa to degradate the hydrocarbon (petroleum Hundill and
eicosane) substrate. Growing test used in two steps, the preculture and culture
step. The biodegradation capacity was measured by quantitative and qualitative
tests. The essay showed an increasing biodegradation capacitypercentage of
bacteria cell mass on hydrocarbon substrate. The percentage on petroleum
Hundill substrat as follows; log phase was 51,6%, descelerate phase was 73%,
and linear phase was 81,4%. On eicosane substrate as follows; log phase was
62,7%, descelerate phase was 85,2%, and linear phase was 85,2%. The qualitative
biodegradation capacity by chromatography result showed separate enchained of
carbon n-alkana in each growth phase on petroleum Hundill substrate. Carbon
chain termination as follows; C11, C12, C14, C15, C16, C18, C22 on log phase,
C12, C17, C19, C20, C24 on descelerate phase, and C12 until C25 even better on
linear phase.
Penulis: Faiqah Umar
Kode Jurnal: jpkimiadd150708
