DEVELOPMENT OF QUALITY CONTROL METHOD FOR GLUCOFARMAKA ANTIDIABETIC JAMU BY HPLC FINGERPRINT ANALYSIS
ABSTRACT: Herbal medicines
become increasingly popular all over the world for preventive and therapeutic
purposes. Quality control of herbal medicines is important to make sure their
safety and efficacy. Chromatographic fingerprinting has been accepted by the
World Health Organization as one reliable strategy for quality control method
in herbal medicines. In this study, high-performance liquid chromatography
fingerprint analysis was developed as a quality control method for glucofarmaka
antidiabetic jamu. The optimum fingerprint chromatogram were obtained using C18
as the stationary phase and linear gradient elution using 10–95%
acetonitrile:water as the mobile phase within 60 minutes of elution and
detection at 210 nm. About 20 peaks were detected and could be used as
fingerprint of glucofarmaka jamu. To evaluate the analytical performance of the
method, we determined the precision, reproducibility, and stability. The result
of the analytical performance showed reliable results. The proposed method
could be used as a quality control method for glucofarmaka antidiabetic jamu
and also for its raw materials.
KEYWORDS: fingerprint
analysis; glucofarmaka antidiabetic jamu; herbal medicines; HPLC; quality
control
Author: Hanifullah Habibie,
Rudi Heryanto, Mohamad Rafi, Latifah Kosim Darusman
Journal Code: jpkimiagg170024
