Identifikasi dan Karakterisasi Senyawa Bioaktif Antikanker dari Ekstrak Etanol Kulit Batang Kayu Bitti (Vitex cofassus)
ABSTRACT: Bitti wood (Vitex
cofassus) is one of the plants in Verbenaceae family and known by the people of
South Sulawesi as the building material. The aims of this research is to identify and characterize the anticancer bioactive compound in ethanol
extract of vortex Bitti wood (Vitex cofassus) and to determine the bioactivity
value. This research uses extraction and fraction method, identification uses
thin layer chromatography (TLC) and phytochemical test to know metabolism
secunder and characterization with FTIR. The result from isolation shows that
the amorf shape with 18 mg has white and yellow colour. The purification is
conduted by using spectroscopy test FTIR. The result shows that the crystal has
flavonoids compound which is solid with phytochemical test like positive
product by using FeCl3 5%, NaOH 10% and H2SO4 P. Thick extract, fraction
combination and amorf continued with toxicity test with the animal test Artemia
salina Leach it uses Brine Shrimp Lethality test (BST) method. LC50 value which
is gotten the three samples is thick extract 29,51 ppm, combination fraction
169,82 ppm and amorf 562,34 ppm.
KEYWORDS: Bitti Wood (Vitex
cofassus); Bioactive Compound; Brine Shrimp Lethality Test (BST); Isolation;
LC50
Penulis: Nuraini, Asriani
Ilyas, Iin Novianty
Kode Jurnal: jpkimiadd150714
