PEROLEHAN GLUKOSA DENGAN HIDROLISIS ENZIMATIS DARI AMPAS TEBU MENGGUNAKAN TRICHODERMA VIRIDE DAN ASPERGILUS NIGER SEBAGAI BAHAN BAKU BIOETANOL
ABSTRACT: Lignocellulosic
biomass is a rich agricultural waste containing cellulose and hemicellulose. Bagasse
(bagasse) is one of the waste biomass containing cellulose and hemicelluloseapproximately
47.7% which can be used to produce bioethanol. Enzymatic process is able to hydrolysis
the polysaccharide compound into its constituent, sugars monomer compound. An enzymatic
hydrolysis process converts cellulose to glucose by using cellulase enzymes.
Some microorganisms producing the enzyme cellulase are Trichoderma viride and
Aspergillus niger.The purpose of this research is to find out comparison of
both these microorganism as a catalyst in the enzymatic hydrolysis process that
produces the highest glucose levels and hydrolysis time. Hydrolysis process is
done by varying the ratio between Tricoderma viride and Aspergillus niger of 1:
0; 0: 1; 0.5: 1 and 1: 0.5 and time sampling 24, 48, 72, 96, 120. Theresults
showed glucose acquisition as much as 360 mg/L with hidrolysis process by
hotplatestirer and 660 mg/L with process by waterbath shaker at composition
ratio of Trichoderma viride: Aspergillus niger 1: 0.5 and the hydrolysis time
is 96 hours.
Penulis: Ellyta Sari, Elly
Desni Rahman, Munas Martynis, Shafira Fiona, dan Junialdi
Kode Jurnal: jpkimiadd150682
