Potensi Ekstrak Etanol Daun Lidah Buaya (Aloe vera L.) Sebagai Antifertilitas Melalui Tampilan Imunohistokimia Caspase 3 Aktif Pada Testis Serta Penilaian Kuantitas dan Kualitas Spermatozoa Mencit (Mus musculus L.)
Abstract: Control of male
fertility is one of the goverment
efforts to reduce the number of population. Several researches have been done
to make a male contraceptive, one of them is using herb to effect male
reproductive system. Aloe vera (Aloe vera L.) has been widely cultivated by the
people and used as materials for food and drugs. One of the benefits of this
plant is it’s potential antifertility. For this research aims to test the
antifertility potential of ethanol extract in Aloe vera (Aloe vera L.) leaf. in
male mice (Mus musculus L.) DDW (Double Ditch Webster) strain. This is an experimental research with complete
randomization. Extract administered orally once a day for four weeks to of 24 male
mice which divided into four groups: control group (0.5 ml/kgBW Aqudest),
treatment group dosage 5mg/kgBW, 10mg/kgBW, 15mg/kgBW extract. We observed the
expression of active caspase-3 microscopically through immunohistochemical
appearance, quantity (number of spermatozoa) and quality (viability of sperm,
sperm morphology and motility). The results were statistically analyzed using
one-way ANOVA followed by multiple comparison (post hoc - Bonferroni test). The
results showed between ethanol extract of Aloe vera leaf with the first dosage
(5mg/kgBW), second dosage (10mg/kgBW) and the third dosage (15mg/kgBW) a
significant reduction of the quantity and quality of spermatozoa (p <0.05).
on the other hand activity of caspase 3 expression active has increased
significantly (p<0.05) in spermatogonial cells of seminiferous tubules
during the administration of ethanol extract of Aloe vera (Aloe vera L.) leaf.
This shows an increase in the incidence of apoptosis during spermatogenesis.
From these results it can be concluded that the ethanol extract of Aloe vera
leaf has potency to be an antifertility drug that can later be developed for
the manufacture of male contraception.
Penulis: Maria Lestari, M.
Pandapotan Nasution, Sry Suryani
Kode Jurnal: jpkimiadd150758
