Produksi dan Uji Aktivitas Enzim Selulase dari Bakteri Bacillus subtilis
ABSTRACT: Enzyme cellulose can
be produced from bacteria Bacillus subtilis. This bacteria is a kind of
bacteria which grounded on the genus that can degrade cellulose to glucose.
Cellulase enzyme acquired different optimum pH and temperature depends on the
bacteria. This research aim to produce and analyze the activity of the
cellulose enzyme from bacteria Bacillus subtilis at optimum pH and temperature.
The research of this enzyme cellulose derived by prolific process and
rejuvenation bacteria Bacillus subtilis
on the nutrition culture, nutrition selective, and nutrition production that
executed by cold centrifugation to obtain extract or the enzyme cellulose at
the temperature of 4°C, 3500 rpm within 15 minutes. On the determine pH with
variety pH 5,6 using buffer acetat and pH 6,0; 6,5; 7,0; 7,5; and 8.0 using
phosphate buffer while on the determine temperature using variety temperature
25°C, 30°C, 35°C, 40°C, 45°C, and 50°C which the next make an experiment
activity enzyme cellulose by method Nelson-Somogy that measured in
spectrophotometer UV-Vis of λ 540 nm. The result shows that the highest enzyme
activity at optimum pH of 6, 0 is 4,3661 10ˉ3 U/mL and in optimum temperature
of 30°C as is 5,6609 10ˉ3 U/mL.
Penulis: Al Maratun Sholihati,
Maswati Baharuddin, Santi Santi
Kode Jurnal: jpkimiadd150718
