STANDARDISASI EKSTRAK PEGAGAN, CENTELLA ASIATICA SEBAGAI OBAT HERBAL TERSTANDAR HEPATOPROTEKTOR
Abstract: Herbal medicinal
products would be affected by the quality of raw materials. In turn, the
quality of raw material will also be influenced by various factors such as soil
conditions, cultivation, post-harvest processing, and the processing of raw
materials into crude drug or extract. Therefore, in order to make good herbal
medicines, it is necessary to make standardization of herbal extracts that
produced herbal medicines that have the same quality and functions of
effectiveness in each process. From preliminary studies that have been done,
Centella asiatica is one of the potential plants as a source of
hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica
extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of
doses body weight and butanol 228.8 mg/kgof doses body weight has been applied
for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective
effects. Ethyl acetate extracts were able to reduce levels of the enzyme
alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and
44 % respectively while butanol extract can reduce the enzymes AST levels by
3%. Standardizationof Centella asiatica extract performed in this study was the
characterization of the extract in the form of non-specific and specific parameters
corresponding to the reference of PPOMN (Ministry of health Republic of
Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate
count microbial contamination, levels of water-soluble compounds, levels of
compounds that are soluble in ethanol, phytochemical test, total phenolic
content, total flavonoid content and the determination of Pb and Cd weight.The
results showed that non-specific parameters for the ethanol extract of Centella
asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes
parameters such as determination of shrinkage on drying ≤ 10%, ash content
≤ 16.6% and negative microbial contamination. Specific parameters for the
ethanol extract of Centella asiatica have met the requirements of Herbal
pharmacopeia in 2008.
Keywords: Centellaasiatica,
hepatoprotective, standardized herbal medicine, specificparameters, and
non-specific parameters
Penulis: Puspa Dewi Lotulung,
Sri Handayani, Teni Ernawati, Tri Yuliani, Nina Artanti, Tjandrawati Mozef
Kode Jurnal: jpkimiadd150652
