VALIDASI METODA FRAP MODIFIKASI PADA PENENTUAN KANDUNGAN ANTIOKSIDAN TOTAL DALAM SAMPEL MANGGA DAN RAMBUTAN
ABSTRACT: Validation of
modified FRAP method to determine total antioxidant content in mango and rambutan
sample have been done. Total antioxidan content in sampel is presented as
Gallic Acid Equivalent (GAE). Modified FRAP method using FeCl3 as oxidizing
agent and orthophenantroline as complexan, which produce complex compounds of
[Fe (C12H8N2)3]+2 sorrelcoloured. Validation parameters are obtained in this
research are r = 0.999, R2 = 0.998, LoD3.13 x 10-6 M and LoQ 1.04 x 10-5 M.
While RSD and recovery in the sample mango andrambutan were 1.15%, 1.34% and
96.31%, 97.72%, respectively. Based on the value of thevalidation parameters,
it can be concluded that this method can be used for determination oftotal
antioxidant content in the mango and rambutan samples.
Penulis: Yefrida, Nor
Ashikina, Refilda
Kode Jurnal: jpkimiadd150677
