Induksi Organogenesis pada Mikropropagasi Anggrek Vanda tricolor Lindl. serta Deteksi Variasi Genetik Hasil Perbanyakan dengan RAPD
Abstract: Induction of
Organogenesis on Micropropagation of Vanda tricolor Orchidand Detection of
Genetic Variation of the Progenies Using RAPD Marker.Research concerning on
micropropagation of Vanda tricolor orchid and detection of geneticvariation
among the progenies has been conducted in the year of 2015. The objective of
theresearch was to obtain the most appropriate explant type for inducing
organogenesis and toobserve genetic variation of the progenies using Random
Amplification of Polymorphic DNA(RAPD). Various pieces of organ from 18 month
old-seedlings were used as material ofmicropropagation, i.e. shoot tip, shoot
base, leaf tip and leaf base. Explants were planted onMS medium without plant
growth regulator. The percentage of explant producing shoots andthe average
number of shoots per-explant were observed at 12 weeks after planting.
Progeniesresulted from the micropropagation were then analysed using RAPD with
5 primers, i.e.OPA8 (5’ GTGACGTAGG 3’), OPA9 (5’ GGGTAACGCC 3’), OPA10 (5’
GTGATCGCAG3’), OPA11 (5’ CAATCGCCGT 3’), OPA13 (5’ CAGCACCCAC 3’). The results
showedthat the most appropriate explant for inducing organogenesis was shoot
base. Analysis ofRAPD showed that the slight variation was detected between
mother plant and the progeniesand also among the progenies. The results
obtained from the current research are promisingand suggests that RAPD markers
can be utilized as a simple molecular tool to assess thegenetic variation of
plants derived in-vitro.
Penulis: RINDANG DWIYANI
Kode Jurnal: jppertaniandd160379
