PENGARUH JENIS PELARUT TERHADAP AKTIVITAS ANTIBAKTERI EKSTRAK DAGING DAN BIJI BUAH BINTARO (CERBERA MANGHAS L.)
ABSTRACT: Bintaro fruit is a
tropical plant that is widely grown in Indonesia, but it has not been utilized
optimally. Seed of bintaro fruit
contains saponin steroids and fatty acids such as palmitat, stearat, oleat,
miristat, linolenat, and linoleat fatty acid.
Kernel of bintaro fruit contains saponin and polifenol that are toxic
for the pests and insects. Kernel and
seed of bintaro fruit may contain antibacterial activity. Fatty acid content of bintrao fruit was
influenced by level of its maturity, more mature contains more fatty acid. The purpose of this research is to determine
the most suitable solvent to produce the best extract which has activity of
anti bacteria and find out the level of anti bacteria activity which is
produced from seed and kernel extracts from the bintaro fruit by using difusi
agar method. This research was arranged
in a Complete Randomized Block Design, two factorial with four
replications. The first factor was
different part of the fruit: seed and kernel.
The second factor was different type of solvent: ethyl acetate, hexane
and aquades. The data were analyzed
using Bartlett test for homogeneity and Tuckey test for additivity. The data then were analyzed using Anova to
see the differences between treatments and tested further using LSD of 5% level
to determine the best treatment. The result of this research indicated that the
kernel of bintaro fruit extracted with ethyl acetate produced the extract which
has the highest resistant zone activity of anti bacteria (about 10,95 mm),
categorized as an average activity of antibacterial, while bintaro seed
extraxted with hexane has less active of antibateria (about 8,43 mm). The most sensitive bacterium againts seed
extracts and kernel of bintaro was Staphylococcus aureus.
Penulis: Samsul Rizal, Hartami
Dewi, Tanto Pratondo Utomo
Kode Jurnal: jppertaniandd151009
