PENGARUH KONSENTRASI ENZIM SELULASE, Α–AMILASE DAN GLUKOAMILASE TERHADAP KADAR GULA REDUKSI DARI ONGGOK
ABSTRACT: Solid cassava waste,
by product of tapioca industries, contains fiber and starch which can be used
as raw materials of bioethanol production.
Both fiber and starch of the cassava solid waste are able to be
enzymatically hydrolyzed into reduced sugar.
The fiber is hydrolyzed with cellulase and the starch is hydrolyzed with
α–amylase and glucoamylase. Objectives
of this research were to find out cellulase, α–amylase, and glucoamylase
concentrations yielding the highest concentration of reduced sugar. To achieve the objectives, the fiber of the
cassava solid waste was hydrolyzed with 5, 10, 15, 20, and 25 FPU cellulase
enzyme at 40oC, pH 4,8, and 200 rpm for
20 minutes. The cassava solid waste,
which has been hydrolyzed with the best concentration of cellulase enzyme, was
then hydrolyzed with 0,58; 1,15; and 1,37 µl α-amylase and 1,1 µl enzyme
glucoamylase/g the cassava waste dry weight.
The result showed that the best cellulase concentration was 25 FPU and
yileded 32,19 mg reduced sugar / 100 mL.
A combination of 1,15 µL
α-amylase dan 1,10 µL glucoamylase/g the cassava waste dry weight was the best
concentration of the enzymes and yielded
62,21 mg reduced sugar/100mL.
Penulis: Sutikno, Marniza,
Nanti Musita
Kode Jurnal: jppertaniandd160869
