PENGARUH PEMBERIAN POLISAKARIDA LARUT AIR BENGKUANG (Pachyrhizus erosus L.) TERHADAP MENCIT PENDERITA DIABETES MELLITUS
ABSTRACT: Yam tuber
(Pachyrhizus erosus L.) is the kind of tuber that contain the most potential
compounds i.e. water-soluble polysaccharide (WSP). The WSP have an ability to
degrade blood glucose level. The purpose of this study was to uncover the
characteristics of WSP in yam and the effect of of the WSP on the male white
mice with diabetes mellitus. The observed parameters on the chemico-pysical
charactersitics of the WSP were viscosity, soluble ability, dextrose equivalent,
polymerization degree, glucose content, total sugars, reducing sugars, and
starch content. In vivo test was using 31 male white mice (aged 2 months) with
an average weight of 25-30 g for glucose tolerance test, body weight measure,
and the hypoglycaemic effect. The glucose tolerance test concisted 5 of mice
and using a Completely Randomize Design (CRD). The blood glucose level was
examined periodic at 30 minutes, 60 minutes, 90 minutes, and 120 minutes. The
examination of body weight and the hypoglycaemic effect was done for 28 mice
using a Completely Randomize Design (CRD) with two factors i.e. the type
treatment: the control group (treated with aquadest), the negative group
(inducted with streptozotocin 180 mg/kg body weight and treated aquadest), the
positive group (treated WSP of 400 mg/kg body weight), and the treatment group
(inducted with streptozotocin of 180 mg/kg body weight and treated with WSP of
400 mg/kg body weight) and periodical time of examination: week-1, week-2,
week-3, and week-4. Each treatment consisted of 7 mice. Glucose tolerance test
showed that the WSP of yam could inhibited glucose absorption in the
gastrointestinal. Result of this research showed that the suplementation of yam
WSP at 400 mg/kg body weight can significantly decreased blood glucose level
and increased body weight (P<0,01) in 4 weeks of streptozotocin induction mice.
Penulis: Sri Rizki Fitri
Kode Jurnal: jppertaniandd160475
