Pengaruh Perlakuan Awal Basa dan Hidrolisis Asam terhadap Kadar Gula Reduksi Ampas Tebu
Abstrak: Bagasse, solid by
product of sugar cane industries, contains high lignocellulose consisting of
46.3% cellulose, 23.0% hemicellulose and 19.7% lignin. The bagasse can be
converted into bioethanol after pretreatment with base and acid and then
fermenting with microbes. The objective of this study was to find out the
effects of sodium hydroxide pretreatment and sulfuric acid hydrolysis on
bagasse reduced sugar. In this study, there were 2 treatments with 3 replications.
The first treatment was submerssion bagasse into 0.50 M NaOH solution at a
temperature of 121oC for 15 minutes. The second treatment was concentrations of
sulphuric acid (H2SO4) that consisted of 5 levels, i.e. 0 M, 0.05 M, 0.10 M,
0.20 M, and 0.30 M. One and an half grams of dried and ground bagasse was put
into 100 mL Erlemeyer flash and then added with 30 mL 1.0 M NaOH solution. The
flash was heated at a temperature of 121oC for 15 minutes. After filtering, the
residue was hydrolyzed with H2SO4 solution. The residue as well as 1.5 g dried
and ground bagasse without pretreatment with NaOH was hydrolyzed with 15 mL
H2SO4 at concentrations of 0, 0.05, 0.10, 0.20, and 0.30 M at a temperature of
121oC for 15 minutes. Filtrates of the solutions were taken to analyze their
reduced sugar content. Reduced sugar of the bagasse samples ranged from 0.05 to
4.20 mg/100 mL. The highest reduced sugar (4.20 ml/mL) was yielded when bagasse
was directly, without treating with NaOH, hydrolyzed with 0.05 M H2SO4 at a
temperature of 121oC for 15 minutes.
Penulis: Sutikno, Novita Sari,
Marniza
Kode Jurnal: jppertaniandd151003
