Transformasi Gen Pembungaan melalui Agrobacterium tumefaciens Secara In-Vitro pada Tanaman Anggrek Vanda tricolor
Abstract: In Vitro
Transformation of Flowering Gene through Agrobacterium tumefaciens on
Vandatricolor Orchid. Research concerning of Agrobacterium-mediated
transformation of Vanda tricolor orchid had been done during March to August
2014. Objectives of this research wasto investigate the effects of period of
inoculation with Agrobacterium suspension and application of acetosyringone (AS)
on the percentage of transforman candidates. The T-DNA harbored the PaFT gene
under the control of promoter of Ubiquitin and the hpt gene, ahygromycine
resistant gene as a selectable marker for transformant selection. This T-DNA
was constructed in the PGAS vector and transferred to the plant genome mediated
by A. tumefaciens. The period of inoculation with Agrobacterium and application
ofAcetosyringone (AS) were trialed in this research. The result showed that a
period of one hour inoculation with Agrobacterium suspension added with 25 ppm
AS resulted in the highest number of transforman candidates. i.e. 7.04%
compared to 4.10% (one hour inoculation without AS), 3.76% (two hours
inoculation without AS), and 4.48% (two hours inoculation with 25 ppm AS).
Keywords: Agrobacterium tumefaciens; acetosyringone; PaFT gene;
period of inoculation; Vanda tricolor orchid
Penulis: RINDANG DWIYANI
Kode Jurnal: jppertaniandd160386
