GROWTH AND MORPHOLOGICAL CHANGES AS AN EARLY INDICATION OF IN VITRO PLOIDIZATION OF Tectona grandis

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ABSTRACT: Ploidization level estimation can be conducted in several methods, through morphological, growth response,  anatomy, cytology, and molecular markers. The simplest and easiest methods are morphological marker and growth response. The study aimed to develop early detection method of polyploidy occurrence in in vitro. Tectona grandis after treated by antimitotic agent colchicine. Nodal segments were immersed at 0, 15, and 30 μM colchicines for 5 days, then cultured for 8 weeks. Observations on plantlet height, number of leaves and morphology were performed at 2, 4, and 6 weeks after planting. Colchicine had high significant effect on the height and significant effect on leaves number. High concentration colchicine inhibited shoot elongation and leaves growth, however it increased morphological changes. The planlets height of 0, 15, and 30 μM of colchicine treatment was 4.14; 3.82; 3.12 cm; while the number of leaves as much as 8.72; 8.4, and 7.5. Colchicine led to increase in morphological changes at the levels 0, 15, 30 μM were 26,60%; 46.66%; and 93.33%. Changes caused by polyploidy differ from media. Changes in polyploidy decreased the height, number of leaves, and induced morphological changes, whereas planting media resulted in vitrification. Response to colchicines in culture of T. grandis plantlet allows the growth and morphology to be a marker for early detection of polyploidization.
Kata Kunci: Tectona grandis; colchicine; polyploidy detection
Penulis: Yusuf Sigit Ahmad Fauzan,  Supriyanto, Teuku Tajuddin
Kode Jurnal: jpkehutanandd170274

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Jp Kehutanan dd 2017