PENGARUH BAP TERHADAP MULTIPLIKASI TUNAS ANGGREK HITAM ( Coelogyne pandurata Lindl ) SECARA KULTUR JARINGAN

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ABSTRACT: Black Orchid is an orchid endemic in West Kalimantan is currently declining presence, so the ex-situ conservation effort are needed. On of the efforts is to plant propagation by tissue culturemethods. This research aimed to get the optimal concentration of Benzil Amino Purine (BAP)for the formation of a black orchid shoot multiplication by tissue culture methods. The researchwas conducted at the Biotechnology Laboratory of the Faculty of Agriculture, University ofTanjungpura. This study used a completely randomized design (CRD). BAP treatment consistsof 6 levels of concentration namely 0 ppm, 0,25 ppm, 0,5 ppm, 1 ppm, 1,25 ppm and 1,5 ppm. The observed variables included the time appeared shoots, number of shoots, and the number ofleaves. The results showed that the concentrations of BAP on MS medium significantly affectedthe entire observation variables. The best concentration for a time appeared shoots and numberof shoots was 1,5 and 1,25 ppm BAP, while the best concentration of BAP for number of leavesis 1,5 ppm.
Keywords: BAP,Coelogyne pandurata, and Tissue culture
Penulis: Nur’Anisa, Reine Suci Wulandari, Asnawati
Kode Jurnal: jpkehutanandd160259

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Jp Kehutanan dd 2016