PENGARUH BAP TERHADAP MULTIPLIKASI TUNAS ANGGREK HITAM ( Coelogyne pandurata Lindl ) SECARA KULTUR JARINGAN
ABSTRACT: Black Orchid is an
orchid endemic in West Kalimantan is currently declining presence, so the ex-situ
conservation effort are needed. On of the efforts is to plant propagation by
tissue culturemethods. This research aimed to get the optimal concentration of
Benzil Amino Purine (BAP)for the formation of a black orchid shoot
multiplication by tissue culture methods. The researchwas conducted at the
Biotechnology Laboratory of the Faculty of Agriculture, University ofTanjungpura.
This study used a completely randomized design (CRD). BAP treatment consistsof
6 levels of concentration namely 0 ppm, 0,25 ppm, 0,5 ppm, 1 ppm, 1,25 ppm and
1,5 ppm. The observed variables included the time appeared shoots, number of
shoots, and the number ofleaves. The results showed that the concentrations of
BAP on MS medium significantly affectedthe entire observation variables. The
best concentration for a time appeared shoots and numberof shoots was 1,5 and
1,25 ppm BAP, while the best concentration of BAP for number of leavesis 1,5
ppm.
Keywords: BAP,Coelogyne
pandurata, and Tissue culture
Penulis: Nur’Anisa, Reine Suci
Wulandari, Asnawati
Kode Jurnal: jpkehutanandd160259
