CARBON TO NITROGEN RATIO AND NITROGENOUS WASTE ACCUMULATION IN THE INTENSIVE CATFISH (Clarias gariepinus) CULTURE

ABSTRACT: This experiment was carried out to determine the optimum C/N ratio for heterotrophic bacteria (biofloc) growth in order to control nitrogenous waste accumulation in thecatfish (Clarias gariepinus) culture. Twenty fish with an initial individual size of about50 g were stocked in fiberglass tanks which were filled with 200 L of water. Fish werefed with commercial floating fish feed with a protein level of 31%-33% (manufacturerlabel). The daily feeding rate was 2.5% of the fish biomass. The inoculation ofcommercial Bacillus sp. isolates was applied in the first day of the experiment afterfish stocking in order to obtain a bacterial density in water of 106 cfu/L. Molases wassuplemented daily to the tanks to adjust C/N ratio in water. Four C/N ratios, i.e. 0, 7,14, and 21, were applied as treatments in this experiment. The results showed that molasses suplementation up to C/N ratio 14 to 21 were able to support the growth ofheterotrophic bacteria and to inhibit the accumulation of total ammonia nitrogen (TAN) and nitrite in water therefore increase water quality for better growth of cultured catfish.

KEYWORDS: C/N ratio, nitrogenous waste, heterotrophic bacteria, biofloc

Author: Bambang Gunadi

Journal Code: jpperikanangg110007

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