DISTRIBUTION ANALYSIS OF ENLARGED CELLS DERIVED FROM GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTED HUMPBACK GROUPER Cromileptes altivelis
Abstract: Characteristic of
Megalocytivirus infection has been known to produce formation of inclusion body
bearing cells (IBCs) on internals organs of fish predominantly on spleen and
kidney. Megalocytivirus that infected grouper is known as Grouper Sleepy
Disease Iridovirus (GSDIV). This study was conducted to answer the effect of entry
sites of GSDIV on distribution of enlarged cells formed on the internal organs
of humpback grouper Cromileptes altivelis. Enlarged cells were observed
histologically under the light microscope on spleen, head kidney, trunk kidney,
liver, gill, heart, stomach, intestine, muscle and brain. Entry sites were
designated to intramuscularly injection, intraperitoneally injection, dipped
gill and inoculum added feed. Enlarged cells were formed on spleen, head
kidney, trunk kidney, liver, gill, heart, stomach, muscle, except on intestine
and brain. All the entry sites resulted in formation of enlarged cells on
spleen, head kidney, trunk kidney, liver, heart. Spleen and head kidney were
the most frequent observed organ. These results suggested that distribution of
enlarged cells were not affected by the entry site of GSDIV.
Keywords: Megalocytivirus;
enlarged cells distribution; internal organs
Author: Indah Mastuti, Ketut
Mahardika
Journal Code: jpperikanangg120029
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