Internal Transcribed Spacer (ITS) as Dna Barcoding to Identify Fungal Species: a Review

Abstract: Despite the fact that fungi are important sources of both bioactive compounds and mycotoxins, and that they are very ubiquitous in our environment, their species identification is hampered by incomplete and often unclear literature. Fungi identification is primarily based on their phenotypic and physiological characteristics. Nowadays, many molecular methods to identify fungal species have been developed. One of the methods considered as a new concept to rapidly and accurately identify unknown fungal sample is DNA Barcoding. This literature review will outline the use of DNA barcoding approach to rapidly identify fungal species and the use of ITS region that recently has been designated as primary DNA barcode for fungal kingdom. “DNA barcode” is a short, highly variable and standardized DNA region with approximately 700 nucleotides in length, which is used as a unique pattern to identify living things. Internal Transcribed Spacer (ITS) region of nuclear DNA (rDNA) has become the most sequenced region to identify fungal taxonomy at species level, and even within species. ITS region is a highly polymorphic non-coding region with enough taxonomic units. Therefore, it is able to separate sequences into species level. Even though ribosomal ITS as a universal barcode marker for fungi is still hampered by few limitations, the ITS will remain as the key choice for fungal identification. The search for alternative regions as DNA marker to improve fungal identification, especially in specific heredities, has already started.

Keywords: DNA barcoding, fungal diversity, Internal Transcribed Spacer (ITS) , molecular identification, universal barcode

Author: Nurrahmi Dewi Fajarningsih

Journal code: jpperikanangg160031

Artikel Terkait :

Jp Perikanan gg 2016

• THE INFLUENCE OF SWIMMING LAYER AND SUB-SURFACE OCEANOGRAPHIC VARIABLES ON CATCH OF ALBACORE (Thunnus alalunga) IN EASTERN INDIAN OCEAN
• MANGROVE OF BERAU: ECOLOGICAL CONDITION, FISHERIES, AND MANAGEMENT OPTIONS
• CURRENT STATUS OF THE POLE-AND-LINE FISHERY IN EASTERN PART OF INDONESIA
• GROWTH COMPARISON OF MAHSEER (Tor tambroides) FROM MANNA AND TARUSAN RIVER IN WESTERN SUMATERA RIVER
• POTENCY AND EFFICACY TEST OF A VACCINE IN ADDITION WITH ADJUVANT AGAINST KOI HERPESVIRUS IN KOI (Cyprinus carpio)
• INDUCTION OF GONADAL MATURATION OF POND CULTURED MALE TIGER SHRIMP, Penaeus monodon WITH DIFFERENT DOSAGES OF GONADOTROPIN RELEASING HORMONE ANALOGUE AGAINST EYE STALK ABLATION
• THE EFFECT OF DEPTH OF HOOKS, SET AND SOAK TIME TO THE CATCH PER UNIT OF EFFORT OF TUNA IN THE EASTERN INDIAN OCEAN
• MORPHOLOGICAL CHARACTERISTICS OF A RED STRAIN OF THE EGYPTIAN AFRICAN CATFISH (Clarias gariepinus BURCHELL 1822)
• STATUS OF SMALL PELAGIC FISHERY IN THE MAKASSAR STRAIT BASED AT THE NORTHERN PART OF JAVA
• BIOLOGY AND CPUE SPATIAL DISTRIBUTION OF ESCOLAR Lepidocybium flavobrunneum (Smith, 1843) IN EASTERN INDIAN OCEAN (EVOLVING FISHERIES: TODAY’S BY-CATCH IS TOMORROW’S TARGET CATCH)
• THE ABILITY OF FAST-GROWING TRANSGENIC AFRICAN CATFISH (Clarias gariepinus) ON PREDATOR AVOIDANCE
• EFFECTS OF DIFFERENT SALINITY LEVELS ON PHYSIOLOGICAL AND HEMATOLOGICAL RESPONSE OF ROCK BREAM Oplegnathus fasciatus
• ROLE OF SUB SURFACE TEMPERATURE, SALINITY AND CHLOROPHYLL TO ALBACORE TUNA ABUNDANCE IN INDIAN OCEAN
• RESPONSE TO SELECTION FOR BODY WEIGHT IN THE THIRD GENERATION OF MASS SELECTION OF THE AFRICAN CATFISH (Clarias gariepinus) AT RESEARCH INSTITUTE FOR FISH BREEDING SUKAMANDI
• GENE TRANSFER ON Betta imbellis THROUGH TRANSFECTION METHOD WITH DIFFERENT DNA CONCENTRATION
• EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio haematopterus
• THE SEASONAL VARIABILITY OF CPUE AND CATCH-AT-SIZE DISTRIBUTION OF TROLL AND HANDLINE TUNA FISHERIES LANDED IN LABUHAN LOMBOK
• Fatty Acid Profile, Carotenoid Content, and In Vitro Anticancer Activity of Karimunjawa and Lampung Sea Cucumber
• Nutritional and Albumin Content of Swamp Fishes from Merauke, Papua, Indonesia
• Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate
• Bioassay-Guided Isolation of an Antibacterial Compound from the Indonesian Soft Coral
• Investigation on Antioxidant Compounds from Marine Algae Extracts Collected from Binuangeun Coast, Banten, Indonesia
• Composition of Fatty Acids in Evaluation of Sea Cucumber Potency for Nutraceutical Product Development
• Purification and Characterization of Transglutaminase from Local Streptomyces Sp. TTA 02 SDS 14
• Ethanolic Fermentation Efficiency of Seaweed Solid Waste Hydrolysates by Saccharomyces cerevisiae