SCREENING OF THERMOSTABLE PROTEASE PRODUCING MICROORGANISMS ISOLATED FROM INDONESIAN HOTSPRING

Abstract: Although many proteases had been studied and characterized, only a few of them are commercially available.  Protease thermostability is one of the crucial properties for industrial application. This research aimed to isolate and to screen the potential isolate which produce thermostable protease. There were 6 isolates (BII-1, BII-2, BII-3, BII-4, BII-6 and LII), isolated using solid Minimal Synthetic Medium (MSM) supplemented with 1.5% skim milk, that have, protease activity. Based on the 16S-rRNA gene sequencing analysis, isolates BII-1, BII-2 and BII- 6 were identified as Bacillus licheniformis, isolates BII-3 and BII-4 were identified as Bacillus subtilis, while isolate LII was identified as Brevibacillus thermoruber. Three isolates (BII-6, BII-4 and LII) were then further investigated for the second screening step using liquid MSM supplemented with 1% skim milk. The isolates (BII-6, BII-4 and LII) optimally produced protease when they were cultivated at 35, 30 and 50o C respectively after 22 h of incubation. Protease produced by BII-6, BII-4 and LII had optimum temperature  of 65, 60 and 85o C, optimum pH at 7-8, 8 and 9 and stable up to 100 min at 55, 60 and 75o C respectively.

Keywords: thermostable protease, Bacillus subtillis, Bacillus licheniformis, Brevibacillus thermoruber

Author: Dewi Seswita Zilda, Eni Harmayani, Jaka Widada, Widya Asmara, Hari Eko Irianto, Gintung Patantis, Yusro Nuri Fawzya

Journal Code: jpperikanangg120009

Artikel Terkait :

Jp Perikanan gg 2012

• SIZE AND FISHING GROUND OFWAHOO (Acanthocybium solandri Cuvier, 1832) FROM CATCH DATA OF TUNA LONGLINE OPERATED IN INDIAN OCEAN
• STOCK ENHANCEMENT IN INDONESIAN LAKE AND RESERVOIRS FISHERIES
• PERFORMANCE OF A FISHERY HARVESTING DIFFERENT MINIMUM SHRIMP SIZES IN THE ARAFURA SEA
• IS THERE ANY RELATIONSHIP BETWEEN FLUCTUATING ASYMMETRY AND REPRODUCTIVE INVESTMENT IN GIANT FEATHERBACK (CHITALA LOPIS, NOTOPTERIDAE)
• EXPLOITATION AND CPUE TREND OF THE SMALL PELAGIC FISHERIES IN THE SULAWESI SEA, INDONESIA
• STOMACH CONTENT OF THREE TUNA SPECIES IN THE EASTERN INDIAN OCEAN
• FISHING ACTIVITY, SIZE DISTRIBUTION, TAG RELEASED AND RECAPTURE OF TUNA TAGGING IN EASTERN INDONESIAN WATERS
• INTERACTION PATTERN BETWEEN FISHERS AND FOREIGN INVESTORS IN THE FISHING BUSINESS AND ITS IMPLICATIONS (A CASE IN NUNUKAN REGENCY, EAST KALIMANTAN)
• CATCH ESTIMATION AND SIZE DISTRIBUTION OF BILLFISHES LANDED IN PORT OF BENOA, BALI
• DEVELOPING RECOMMENDATIONS FOR UNDERTAKING CPUE STANDARDISATION USING OBSERVER PROGRAM DATA
• THE GENETIC STRUCTURE OF THE WHITE CYPRINID (TOR TAMBROIDES) POPULATIONS BASEDON COIGENE SEQUENCE ANALYZES
• ENVIRONMENTAL CONDITON, FISH RESOURCES AND MANAGEMENT OF MANINJAU LAKE OF WEST SUMATERA
• INVESTIGATION ON BIODIVERSITY AND CONSERVATION OF FRESHWATER FISHES IN INDONESIA
• VERTICAL-HORIZONTAL WATER QUALITY PROFILES OF BATUR LAKE, BANGLI DISTRICT, BALI SUPPORTING SUSTAINABLE LAKE MANAGEMENT
• DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR
• HISTOLOGICAL STUDY ON INTERNAL ORGANS DEVELOPMENT OF MANGROVE SNAPPER, Lutjanus argentimaculatus LARVAE
• EFFECTS OF CURRENT VELOCITY ON FILTRATION AND INGESTION RATE OF PEARL OYSTER (Pinctada maxima)
• THE DIGESTIVE TRACT DEVELOPMENT OF THE TIGER GROUPER Epinephelus fuscoguttatus LARVAE TREATED WITH THYROXINE HORMONE
• STUDY OF VERTEBRAL MORPHOGENESIS OF COBIA LARVAE, (Rachycentron canadum) BY DOUBLE STAINING METHODS
• EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE
• PROPOLIS, THE ALTERNATIVE NATURAL MATERIAL FOR SEX REVERSAL IN TILAPIA
• THE SURVIVAL OF TRANSPLANTED CORAL ON PYRAMID-SHAPED FISH SHELTER ON THE COASTAL WATERS OF KELAPA AND HARAPAN ISLANDS, KEPULAUAN SERIBU, JAKARTA
• THE DYNAMIC OF LANDSCAPE CHANGE AT COAST AREA, IN LABAKKANG SUBDISTRICT PANGKEP REGENCY, SOUTH SULAWESI
• SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV)
• DISTRIBUTION ANALYSIS OF ENLARGED CELLS DERIVED FROM GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTED HUMPBACK GROUPER Cromileptes altivelis