BIOETHANOL PRODUCTION FROM SEAWEED PROCESSING WASTE BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF)
Abstract: Seaweed processing
waste has been used for bioethanol production through simultaneous
saccharification and fermentation (SSF). SSF is commonly used for bioethanol
production to shorten the process and to increase the yield of ethanol produced
by Trichoderma reesei and Saccharomyces cerevisiae. The aim of this research
was to obtain the best concentration of T. reesei and S. cerevisiae to produce
bioethanol by SSF. The concentration of T. reesei and S. cerevisiae used was 0
(control), 5, 10, 15 and 20% (v/v). The SSF process was carried out by using
shaking incubator at 35 °C and rotation of 150 rpm for 3 days. The untreated
and hot water treated seaweed processing waste used in this study have moisture
content values of 12.94±0.08% and 15.38±0.19%, ash content values of
16.72±0.08% and 18.39±0.19%, lignin content values of 15.38±0.11% and
12.74±0.38%, and cellulose content values of 26.92±0.57% and 34.57±0.81%,
respectively. The result of SSF process of seaweed processing waste showed that
different concentrations of T. reesei and S. cerevisiae (control, 5, 10, 15 and
20%) yielded significant effect (p<0.05) on the total reducing sugars and
ethanol produced. The Duncan Multiple Range Test (DMRT) showed that the
treatment 10% of T. reesei and S. cerevisiae concentration in the seaweed
processing waste treated with hot water was the best treatment producing
highest yield of ethanol.
Keywords: bioethanol,
treatment, seaweed processing waste, SSF
Author: Andi Hakim, Ekowati
Chasanah, Uju Uju, Joko Santoso
Journal Code: jpperikanangg170003
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