The Identification of Carotenoids and Testing of Carotenoid Antioxidants from Sand Lobster (Panulirus homarus) Egg Extract
Abstract: Sand lobsters
(Panulirus homarus) are organisms that play an important role in supporting a
society’s economy. Lobsters have a business appeal due to their high demand,
enjoyable taste, as well as significant nutritional content. It is interesting
that sand lobster eggs have a yellow orange color as an indicator of the
presence of carotenoid content. Related with advancements in research and the
utilization of carotenoids, this research identifies carotenoids and tests
carotenoid antioxidants from sand lobster (Panulirus homarus) egg extract. In
identifying carotenoids in sand lobster egg extract, HPLC (high performance
liquid chromatography) (shimadzu LC-10AD, Japan) is used. In testing carotenoid
antioxidants from sand lobster eggs, a DPPH method is utilized. The
identification results with HPLC reveal that the sample contains carotenoids.
The kinds of carotenoids identified are dinoxanthin, diadinoxanthin,
zeaxanthin, lutein, astaxanthin, and violaxanthin. Among the six kinds of
carotenoids identified, lutein is the type of carotenoid that has the highest
concentration. These carotenoid compounds are detected in wavelengths of
400-500 nm. The test results of antioxidant power reveal that to hamper free
radicals (IC50), a sample concentration of 6675.25 µg.ml-1 is needed. The
ability to hinder free radicals from a sample extract is mostly conducted by
lutein, zeaxanthin, and astaxantin. If seen from the analytical results, it can
be surmised that sand lobster eggs contain carotenoids that can be used as a
carotenoid source for humans.
Keywords: Sand Lobster, HPLC,
Carotenoids, DPPH, Antioxidant
Author: James Ngginak, Jubhar
C Mangibulude, Ferdy S Rondonuwu
Journal Code: jpperikanangg170050
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