Correlation between Carcinoma Percentage (CP) and Lymphatic Microvessel Density (LMVD) Based on D2-30/Podoplanin as Metastatic Prognostic Factor to Lymph Nodes in not Otherwise Specified (NOS) Colorectal Adenocarcinoma
Abstract: In colorectal
carcinoma (CRC), myofibroblast are the main component cells in tumour stroma
which have an important role in the metastases process. The precentage between
carcinoma and desmoplastic stroma known as carcinoma percentage (CP), can be
used as an independent predictor metastases. D2-40/Podoplanin (PDPN) known as a
spesific marker for lymphatic endothelial cell (LEC), which used to assess
lymphatic microvessel density (LMVD) and lymphatic vessel invasion (LVI). This
study aims to determine correlation and association between CP, LMVD and LVI
with the metastases process to lymph node (LN).
Method: CP assessment conducted on 44 samples of adenocarcinoma not
otherwise specified (NOS) colorectal were divided into 22 cases with CP-High
and 22 cases with CP-Low and examination D2-40/Podoplanin to assess LMVD and LVI.
The statistical test is performed to find the correlation between CP and LMVD,
as well as the relationship between CP, LVI and metastasis KGB.
Results: There were a strong correlation between CP and LMVD intratumoral
and peritumoral area with the negative correlation. There were a significant
association (p=0,00) between LMVD (intratumoral and peritumoral area) with the
LVI. There was a significant association between LVI and lymph node metastases
(p = 0,03). Intratumoral area showed significant association with lymph node
metastases (p = 0,04), whereas peritumoral area showed no significant
association (p = 0,17).
Conclusion: CP examination in histopathology specimen can be used to
predict high/low rate of tumour cells metastases to the lymph node, based on a
strong correlation between CP and LMVD.
Keywords: carcinoma
percentage; lymphatic microvessel density; lymphatic vessel Invasion; lymphatic
endothelial cell
Author: Elisabeth Indria Sari,
Ening Krisnuhoni, Puspita Eka Wuyung
Journal Code: jpkedokterangg170376
