Analisis Profil Protein Ekstrak Aquades dan Etanol Daun Mimba (Azadirachta Indica A. Juss) dengan Metode SDS-PAGE
ABSTRACT: Neem leaf is used to
increase body imune response, antivirus, antifungi, antibacterial, dan
antioxidant. These biology activities was suspected because of proteinin neem
leaf. Protein in plants can be used as pharmacogenetic identification becausesome
proteins in plants posse characteristic can defense to some pathogen mcroorganism.
The way to know protein in the organism is using profile protein analytic through
SDS-PAGE method. SDS-PAGE separates protein molecules by size and particular
shapping and also can determine protein molecular weight. Protein profiles can
be obtained with protein extracts using solvents. Protein is a polar compound
which is bonded to a polar solvent therefore chosen ethanol and aquadest as a
liquid asextraction solvent . Purpose: To know the protein profile of aquadest
and ethanol extracts of neem leaf by using SDS-PAGE. Method: This study uses
neem leaf as a sample, aquadest and 70% ethanol as the extraction solvent then
analyzed using SDSPAGE. Result: The results showed 9 protein bands on the
aquadest extract of neemleaf and 8 of protein bands on the ethanol extract of
neem leaf. Conclusion: From the study, there is a difference between the amount
of protein bands aquadest extracts ofneem leaf with ethanol extract of neem
leaf.
Keywords: aquadest extract,
ethanol extract, neem leaf, protein profile, SDS-PAGE
Penulis: Miftah Dewi Masyitoh
Kode Jurnal: jpkesmasdd160525
