Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kadar Malondialdehid Hepar Tikus Wistar yang Diinduksi DMBA
ABSTRACT: DMBA
(7,12-Dimethylbenz(α)antracene) is a prototype of Polycyclic Aromatic
Hydrocarbons (PAHs) which is present in automobile exhaust, cigarette smoke,
and wood stoves. DMBA is converted into its reactive metabolites DMBA-DE and
cause liver cell membrane lipid peroxidation, which results in an increased of
liver malondialdehyde (MDA) levels. Broccoli contains flavonoid that able to
prevent lipid peroxidation. The current study was conducted to find out the
different hepatoprotective effect of ethanolic extract of broccoli against
DMBA-induced hepatotoxicity in wistar rats using liver MDA as a marker. A total
of 24 male wistar rats were divided into six groups, consisting of normal
control (distilled water), negative control (DMBA), and treatment (ethanolic
extract of broccoli in doses of 250, 500, 1000, and 2000 mg/kgBW). Rats were
treated for 7 days prior to a single dose of DMBA on the 8th day of treatment.
Liver tissue samples were taken on the 12th day for measurement of liver MDA
levels. Data were analyzed using One Way ANOVA followed by Post Hoc Test LSD.
The present study showed significant differences on liver MDA levels between
groups (p<0.001). This study concluded that there were different
hepatoprotective effects of ethanolic extract of broccoli treatment on liver
MDA levels in wistar rats induced by DMBA.
Keywords: ethanolic extract of
broccoli, DMBA, malondialdehyde, hepatoprotective
Penulis: Dear Farah Sielma
Kode Jurnal: jpkesmasdd160512