Pengaruh pemberian ekstrak daun Alfalfa (Medicago sativa) terhadap profil lipid dan kadar malondialdehida tikus hiperkolesterolemia
Abstract: Hypercholesterolemic
as an indicator of cardiovascular disease is a major cause of morbidity and
mortality in the world. Increased oxidative stress in hypercholesterolemic
triggers lipid peroxidation effect directly on the cell membrane damage and
initiate various diseases. The use of natural materials with relatively few
side effects is an hypercholesterolemic management alternative. Experimental
studies showed that Alfalfa leaf extract had an effective antioxidant activity.
Objective: To determine the effect of Alfalfa leaf extract on serum
lipids and malondialdehyde (MDA) level of hypercholesterolemic rats.
Method: Pre-posttest randomized control group used twenty-eight male
Sprague-Dawley rats were randomly divided into four groups,
hypercholesterolemic without treatment (control), hypercholesterolemic with
extract at dose 20 mg/200 g/d (X1), dose 40 mg/200 g/d (X2), dose 60 mg/200 g/d
(X3) for 21 days after the rats got hypercholesterolemic. Serum lipid was
measured by CHOD-PAP method and level of MDA plasma was measured by TBARS
method. A hypothesis test was analyzed by One Way ANOVA continued by Post hoc LSD
test.
Results: Alfalfa extract significant decrease in serum total cholesterol
(114.18±3.0 mg/dl; p<0.001). LDL cholesterol level was the lowest in group
X3 (45.26±6.03 mg/dL; p<0.001), and triglycerides level was the lowest in
group X3 (77.33±2.69 mg/dL; p<0.001). There were no differences in MDA
plasma level was the lowest in the group X3 (2.07±0.09 nmol/ml; p<0.001) and
a significant increase in serum HDL cholesterol (43.21±7.80 mg/dL; p<0.001).
Conclusion: The treatment of Alfalfa (Medicago sativa) leaf extract gives
an effect of lower total cholesterol, LDL cholesterol, triglycerides, MDA and
gives an effect higher HDL cholesterol.
Keywords: Alfalfa;
hypercholesterolemic; lipid profile; MDA
Penulis: Joyeti Darni,
Kusmiyati Tjahjono, Muchlis Achsan Udji Sofro
Kode Jurnal: jpkesmasdd160587
