Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi
Abstract: High-fat diet
increases the levels of fat, especially cholesterol and triglycerides that
cause hyperlipidemia. Polyunsaturated fatty acids are very easily oxidized by
free radicals reactive oxygens species (ROS) called lipid peroxidation, to
produce compounds that malondialdehyde (MDA), is toxic and can damage plasma
membrane and liver tissue. Mung bean sprouts are known to contain antioxidants
which act to inhibit lipid peroxidation process.
Objective: Determine the effect of mung bean sprouts to the level of
plasma MDA and liver tissue of rats with high-fat diets.
Method: This research is an experimental study with an only post-test
design with the control group. Thirty-five male Sprague-Dawley rats were
divided into 5 groups. Group K1 received fed standard; K2 received high-fat
diet without mung bean sprouts. Group P1 received high-fat diet and mung bean
sprouts dose of 0.5 mL/gBW. Group P2
received high-fat diet and mung bean sprouts dose of 1 mL/gBW. Group P3
received high-fat diet and vitamin E doses of 23 IU. All group were treated for
28 days. Analysis of MDA plasma and liver tissue performed after treatment.
Results: Group K1 has plasma MDA levels of 1.17 ± 0.14 nmol/mL Mean MDA
plasma level in the group K1 Group of K1 Mean MDA plasma level in the group K2
(39.7±0.19 nmol/mL) was higher than the group of K1 (1,17±0,14 nmol/mL), group
P1 (2.94±0.09 nmol/mL), group P2 (1.73±0.08 nmol/mL) and group P3 (1.53±0.07
nmol/mL). Group K1 has liver tissue MDA Levels of 1,64±0,11 nmol/g. Mean MDA
liver tissue level in the group of K2 (4.90±0.29 nmol/g) was higher than the
group of P1 (3.68±0.45 nmol/g), group P2 (2.27±0.10 nmol/g), and group P3
(2.01±0.11 nmol/g).
Conclusion: Level of MDA plasma and liver tissue of rat in group P1 and
P2 was lower than a level of MDA plasma and liver tissue of rat in group K1,
but higher than the level of MDA plasma and liver tissue of rat in group P3.
There was a very strong correlation between the level of MDA plasma and liver
tissue of rat was given the mung bean sprouts and high-fat diet.
Keywords: high fat diet; MDA
plasma; mung bean sprouts; vitamin E
Penulis: Novidiyanto
Kode Jurnal: jpkesmasdd160594
