Uji Sitotoksisitas dan Proliferasi Senyawa 1-(4-nitrobenzoiloksi- metil)-5-fluorourasil terhadap Sel Kanker Payudara MCF-7
ABSTRACT: 1-(4-nitrobenzoyloxymethyl)-5-fluorouracil
(4-NFU) is a derivative of anticancer drug 5-fluorouracil (5-FU) which has been
substituted by alkyl, ester, benzene, and nitro groups at N1 position. 4-NFU
has been synthesized by Kurnia but anticancer study has not been reported. This
research aims to compare the cytotoxicity and antiproliferative activity
between 4-NFU and 5-FU on MCF-7 cells. The cytotoxicity and antiproliferative
activity were studied by MTT method. The cytotoxicity study was done for 24
hours. The antiproliferative study was done for 24, 48, and 72 hours. The
result showed that cytotoxicity activity of 4-NFU (IC50 = 134.039 μM) was
higher than 5-FU (IC50 = 4211.508 μM) on MCF-7 cells. Based on the unpaired t
test, there was a significant difference between IC50of 4-NFU and 5-FU
(α<0.05). The antiproliferative study showed that there was a growth
inhibition on MCF-7 cells. The doubling time value of 4-NFU was 290.51 hours at
½ IC50; 350.97 hours at IC50; and 418.2 hours at 1½ IC50. The doubling time
value of 5-FU was 185.99 hours at ½ IC50; 214 hours at IC50; and 220.63 hours
at 1½ IC50. The doubling time value of 4-NFU was higher than 5-FU so that
antiproliferative activity of 4-NFU was higher than 5-FU on MCF-7 cells.
Keywords:
1-(4-nitrobenzoyloxymethyl)-5-fluorouracil, cytotoxicity, proliferation, MCF-7
Penulis: Eka Mustika Wati
Kode Jurnal: jpkesmasdd160513
