Aktivitas Ekstrak Metanol Bawang Putih (Allium sativum) sebagai Penghambat Pembentukan Biofilm pada Pseudomonas aeruginosa
ABSTRACT: Garlic (Allium
sativum) is one of the most important agricultural product that has
organosulphur compound, such as alliin, allicin, and ajoene. Those organosulphur compound used as
antibiofilm agent by inhibiting adhesion and quorum sensing process in
Pseudomonas aeruginosa. The purpose of study was to identify the antibiofilm
effect and Minimum Biofilm Inhibitory Concentration (MBIC) of methanolic
extract of garlic against the growth of P. aeruginosa biofilm. This was quasi
experimental with posttest only control group design. The colony of P.
aeruginosa were divided into 7 groups with 5 groups used methanolic extract of
garlic as P1-P5 at a dose 0,3; 0,6; 0,9;1,2; and 1,5 mg/mL, positive control
group (K+) used NAC 80 mg/mL, and negative control group (K-) used sterile
water. This research using Microtitter Plate Biofilm Assay Method with 4
replication. The absorbance value was analized by using One Way Anova
statistical analysis, it showed the significance value p=0,000 (p<0,05). By
using linier regression, the MBIC was obtained at the dose of 0,3468 mg/mL. In
conclusion, methanolic extract of garlic had ability to inhibit the growth
biofilm of P. aeruginosa.
Keywords: biofilm, garlic,
Minimum Biofilm Inhibitory Concentration, P. aeruginosa
Penulis: Risty Pradana Linggan
Wangi, Enny Suswati, Desie Dwi Wisudanti
Kode Jurnal: jpkesmasdd170107
