Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kerusakan Histologis Sel Hati Tikus Wistar yang Diinduksi DMBA
ABSTRACT: DMBA
(7,12-Dimethylbenz(α)antracene) is one of Polycyclic Aromatic Hydrocarbon’s
form that is abundant on air pollution, i.e. automobile exhaust, cigarette
smoke, and wood stoves. DMBA is metabolized in liver result in DMBA-DE. DMBA-DE
is a free radical compound that has hepatotoxic effect. Broccoli contains
flavonoid that has antioxidant effect. It can stabilize DMBA-DE radical
compound. This research was conducted to investigate the hepatoprotective
effect of ethanolic extract of broccoli on the damage of rat’s liver cells
induced by DMBA. This research used 30 white male rats that was divided into 6
groups: normal control (aquadest), positive control (DMBA) and the treatment
groups(using brocolli ethanolic extract with dose : 250, 500, 1000, and 2000
mg/kgBB). The treatment was given for 7 days followed by single-dose DMBA 15
mg/kgBB induction in the 8th day. Histological appearance of liver cells were
examined on the 12th day. Data were analyzed by using Kruskal Wallis test
followed by Mann Whitney. The result showed that there was a significant
difference among the groups with p<0.05. In conclusion, there were some
differences of hepatoprotective effect of broccoli ethanolic extract on the
histologically damage of rats liver cells induced by DMBA.
Keywords: ethanolic extract of
broccoli, DMBA, histopathology, hepatoprotective
Penulis: Muhtar Ady Kusuma,
Elly Nurus Sakinah, Rosita Dewi
Kode Jurnal: jpkesmasdd170036
