Efektivitas Chlorhexidine sebagai Inhibitor MMP-2 dan MMP-9 dalam Proses Dermonekrosis Akibat Racun Kumbang Tomcat (Paederus sp.)
ABSTRACT: Paederus dermatitis
is dermonecrotic condition caused by Rove beetles (Paederus sp.). Paederin
toxin come from endosymbiotic between rove beetles and Pseudomonas aeruginosa
and can produces MMP-2 and MMP-9. Chlorhexidine is expected to be alternative
therapy and prevention of dermatitis paederus due to it's MMP inhibitor and
bacteriostatic effect. The study aimed to investigated the effectivity of
chlorhexidine as MMP-2 and MMP-9 inhibitor in dermonecrotic process due to rove
beetles toxin. Research groups consisted of negative control (paederin toxin),
treatment 1 (paederin toxin + chlorhexidine 0,012%), treatment 2 (paederin
toxin + chlorhexidine 0,06%), treatment 3 (paederin toxin + chlorhexidine
0,3%), and normal control (aquadest). As much as 9,3 µg paederin toxin and
chlorhexidine are injected to the well of 8% acrylamide gel containing gelatin.
Measuring of band density using ImageJ showed a decreasing of MMP-2 and MMP-9
band density. Negative control group was considered to have 1 of relative
optical density. The result showed that chlorhexidine 0,06% can inhibits 21,6%
MMP-2 activities and at 0,3% can inhibits 16,4% MMP-9 activities. One way ANOVA
test show different significantly between negative control and treatment group
(p<0,05). In conclusion chlorhexidine has potency as MMP-2 and MMP-9
inhibitor in dermonecrotic process due to Rove Beetles toxin.
Keywords: Rove
beetles(Paederus sp.), dermonrcrotic, MMP-2, MMP-9, chlorhexidine
Penulis: Putri Erlinda
Kusumaningarum, Al Munawir
Kode Jurnal: jpkesmasdd170031
