Efektivitas Kombinasi Vankomisin dan Vitamin C terhadap Pertumbuhan MRSA (Methicillin Resistant Staphylococcus aureus)
ABSTRACT: Nephrotoxicity due
to prolonged use of vancomycin becomes a big issue in patients with MRSA
infections and the use of vitamin C has been proved to decrease this side
effect. Vitamin C with its combination with antibiotic will halt MRSA growth by
forming Reactive Oxygen Species (ROS). This study aimed to determine the effect
of vancomycin and vitamin C combination in order to halt the MRSA growth and
the minimum concentration of vitamin C which can decrease the Minimum
Inhibitory Concentration (MIC) of vancomycin. This study used a quasi
experimental design with 7 groups, 1 as negative control which only given
vancomycin and 6 other groups which given vancomycin and vitamin C with
following concentration 0,4; 0,8; 1,6; 3,2; 6,4; and 12,8 mg/mL. We used microdilution
method with 4 repetitions to measure the MIC value. This study revealed that
there were no difference between control and other groups, the MIC of
vancomycin is 1 µg/mL. Combination effect was measured by Fractional Inhibitory
Concentration Index (FICI) calculation which showed indifferent effect to all
groups. This study concluded that combination of vancomycin and vitamin C
effect has no difference with control group and by adding vitamin C until 12,8
mg/mL did not decrease MRSA growth.
Keywords: MRSA, vancomycin,
vitamin C, Minimum Inhibitory Concentration (MIC)
Penulis: Ulin Nuha Jazmin,
Dini Agustina, Rony Prasetyo
Kode Jurnal: jpkesmasdd180025
