Isolasi Dan Karakterisasi Flavonoid Antioksidan Dari Herba Meniran (Phyllanthus niruri L.)
Abstrak: The isolation of
flavonoid substance from Meniran has been performed. Of 100 grams of Meniran’s
dried powder, 4.286 grams (4.286%) of thick extract, 1.76 grams of n-hexane
fraction, 1.91 grams of CHCl3 fraction, 1.8 grams ofethyl acetate fraction,
2.12 grams of butanol fraction and 24.86 grams of residual fraction has been
derived. After preparative chromatography,which is to isolate flavonoid, had
been performed, it acquired four blotches which were marked as A,B,C and D. And
afterwards it was evaporated which resulted 26.9 mg of flavonoid A, 30.4 mg of
flavonoid B, 34.6 mg of flavonoid C and 35.4 mg of flavonoid D.
Characterization was focused on flavonoid C as it has the highest antioxidant
activities which was recognized from its high inhibition persentage which was
attained following a DPPH test. flavonoid C is amorf, yellow coloured, and
dissipated at 161.8o – 163.2o C. Based on UV spectrum with slide reactor and
infrared spectrum, it was assumed that flavonoid C is an aglicone isoflavone
which has an –OH on the 5th position and an o-diOH in ring A (6.7 or 7.8).
Kata Kunci: preparative
chromatography, flavonoid, Phyllanthus niruri L.
Penulis: Harrizul Rivai, Diyah
Permata Sari, Zet Rizal
Kode Jurnal: jpfarmasidd120275
