Pengembangan Sensor Kloramfenikol Berbasis Imobilisasi Bovine Serum Albumin (BSA) pada Selulosa Asetat dengan Metode Spektroflorometri
ABSTRACT: The chloramphenicol
usage in shrimp cultivation is an example of antibiotics abuse that have
adverse effects for health. According to Indonesian Drug and Food Agency (BPOM)
survey, it showed that from 14 samples of shrimp test, entirely contain
residues of chloramphenicol. In this context, the sensor has been developed
base on cellulose acetate membrane immobilized with bovine serum albumin (BSA).
The chloramphenicol sensor has a linearity range of 2-12 μg/ml and the r value
of -0.997. The value of limit of detection is 0.157 μg/ml and limit of
quantification is 0.472 μg/ml. The RSD value of repeatability is 1.542 % and
the value of intermediate precision is 1.058%. The average value of recovery as
the parameter an accuracy test of 95.338 ± 0.636% and this sensor has a good selectivity
tests to erythromycin, pellets and shrimp meat. The result also shown in good
agreement with the conventional spectrofluorometry method
Keywords: shrimp,
chloramphenicol, BSA, sensor, spectrofluorometry
Penulis: Dhany Alghifari,
Bambang Kuswandi, Dwi Koko Pratoko
Kode Jurnal: jpkesmasdd170038
