Pengembangan Sensor Kloramfenikol Berbasis Bovine Serum Albumin menggunakan Spektrofotometri UV
ABSTRACT: Misuse of
antibiotics has been discovered in shrimp exported from Indonesia, one of them
is chloramphenicol, therefore it is necessary to develop methods for
chloramphenicol detection. In this study, the chemical sensor for
chloramphenicol detection has been developed. The sensor developed by
immobilized bovine serum albumin (BSA) onto cellulose acetate membrane. The
sensor has linear range of 6-16 μg/ml chloramphenicol with r 0.999 and limit of
detection (LOD)-limit of quantification (LOQ) value of 0.74-2.21 μg/ml. The RSD
value of repeatability and intermediet precision were 2.41% and 2.31%, where the
recovery was 100.29%. Three of the five samples of tiger shrimp on the market
containing chloramphenicol at level of 1.03; 2.48; and 3 μg/ml. The result also
shown in good agreement with conventional spectrophotometry UV-Vis method.
Keywords: shrimp,
chloramphenicol, BSA, cellulose acetate
Penulis: Sarah Aisha, Bambang
Kuswandi, Dwi Koko Pratoko
Kode Jurnal: jpkesmasdd180009
