Potensi selada air (Nasturtium officinale R. Br) sebagai antioksidan dan agen anti proliferasi terhadap sel MCF-7 secara in vitro
Abstract: This study aimed to
determine the potential of watercress extract as an antioxidant and
antiproliferation against cancer cells in vitro. Cells used in this study were
breast cancer culture cells (MCF-7) and vero cells. Watercress was extracted
using water solvent and 70% ethanol. Phytochemical test was performed on both
types of extract. Toxicity test was measured using BSLT method, assessment of
antioxidant activity was measured using DPPH method and inhibitory
proliferation in cancer cell was measured using MTT Assay method. Analysis of
the data using ANOVA, if there is a significant difference between treatments
will be followed up with Duncan test. The results of phytochemical tests showed
that watercress extracts had bioactive content namely flavonoids, tannins,
saponins, and steroids. The result of BSLT test showed that the strongest
extract toxicity was 70% ethanol extract with LC50 value of 406.02 ppm and had
the highest antioxidant activity with IC50 value of 102.26 ppm, while
proliferation inhibitory test found in MCF-7 breast cancer cells had IC50 value
of 1.696 μg / ml and not toxic to vero cells. Result showed that the ethanolic
extract of Nasturtium Officinale gave a significant effect on MCF-7 breast
cancer cells inhibition (p=0,015) at concentration 50, 400, 800 and 1000 ppm.
The mechanism of proliferative inhibition was associated with phytochemical
content present in watercress.
Keywords: antioxidant; cancer
cell; nasturtium officinale; phytochemical
Penulis: Dewi Rahmayani
Rahman, Rimbawan, Siti Madanijah, Sri Purwaningsih
Kode Jurnal: jpkesmasdd170489
