Activity Tests of Bioactive Material of Salung Leaf (Psychotria viridiflora Reinw. Ex. Blume) against Salmonella thypi Bacteria In Vitro And In Vivo
Abstract: Activity test of
bioactive material of Salung leaf (Psychotria viridiflora Reinw. ex. Blume)
against Salmonella thypi in vitro and in vivo has been carried out. Bioactive
material was obtained from the maceration and followed by fractionation of
liquid-liquid fractionation. Antibacterial activity test performed in vitro to
determine the value of the minimum inhibitory concentration (MIC) and in vivo
to determine the ability of bioactive cure diarrhea in in rats (Rattus
norvegicus) infected by Salmonella typhi. Treatment of bioactive material given
is 0, 10, 50 and 100 mg kg-1 of weight. The results showed that the MIC of
salung leaf’s bioactive material to Salmonella typhi was 250 μg mL-1. Bioactive
ingredient at dose of 10 and 50 mg kg-1 were able to decrease the number of
bacterial colonies to 4.14x106 cfu g-1 and 5.4x105 cfu g-1, less than 5.04x106
cfu g-1 as control. Bioactive material in weight of 100 mg kg-1 of weight could
reduce the population of Salmonella typhi to zero after 12 days of treatment.
The ability to reduce the amount of bacterial colonies of the bioactive
material 100 mg kg-1 of weight is equal to the ability of positive control
chloramphenicol 10 mg kg-1 of weight.
Keywords: antibacterial,
bioactive material, Psychotria viridiflora, Salmonella thypi, Rattus norvegicus
Penulis: SALNI
Kode Jurnal: jpfarmasidd160429
