AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI AKAR MANGROVE (Rhizophora apiculata Blume) SECARA KLT BIOAUTOGRAFI
Abstrak: Endophytic fungi have
the ability to produce bioactive compounds one of which is antibacterial. A
search of bioactive compounds in this research are the first was isolated
endophytic fungi on the roots of mangrove then be purified and macroscopic
order to obtain pure 6 isolates endophytic fungi. Proceed with the screening
test to test bacteria and endophytic fungal isolates obtained first by IJM 2
code which provides activity against 5
test bacteria with the formation of the largest inhibition zone diameter. Then
proceed with the fermentation of isolates IJM 2 for 21 days at room temperature
using Potato Dextrose Broth medium after the separation to obtain a supernatant
and mycelia. Then proceed with the solvent extraction using ethyl acetate and
the extract obtained extract supernatant and mycelia. The extract obtained is
then followed by TLC-Bioautografi testing using the eluent n-butanol: acetic
acid: water (8: 1: 2). Results chromatogram profile with KLT-Bioutografi method
showed antibacterial activity against bacteria Shigella dysentriae Pseudomonas
aeruginosa, Salmonella thypii, Vibrio cholerae, Stahpylococcus aureus.
Keywords: Endophytic fungi,
antibacterial, Rhizophora apiculata Blume
Penulis: Fitriana, Eka
Nurshitya
Kode Jurnal: jpfarmasidd170392
