Aktivitas Antioksidan Fraksi Rimpang Kencur (Kaempferia Rhizoma) Dengan Menggunakan Metode Peredaman 1,1 Diphenyl-2-picrylhydrazil (DPPH)
Abstrak: Kaempferia is one of
the most widely used plants in society is a traditional at the medicine.
Empirically Kaempferia rhizome has a special quality as a wound healer, good
for digestion, antibacterial, and as an antioksidant. Based on research been
conducted that Kaempferia extract has antioxidant activity. This research aimed
to test antioxidant activity base on IC50 value on kaempferia rhizome fraction
by the reduction method 1,1 diphenil-2-phicrylhydrazil (DPPH). The extract
obtained was fractionated using a vacuum liquid chromatography method, then
performed free radical reduction meansurements using UV-VIS spectrophotometry.
A positive fraction of antioxidants activity is the n-hexane fraction : ethyl
acetate (7:3 and 8:2). The n-hexane fraction 8:2 showed higher antioxidant
activity with IC50 731.832 µg/mL. The
n-hexane fraction: ethyl acetate 7:3 with IC50
829.737 µg/mL
Kata Kunci: Kaempferia
Rhizoma, antioxidant, DPPH, IC50
Penulis: Zahrah Muhafidzah,
Seniwati Dali, Rezky Amriati Syarif
Kode Jurnal: jpfarmasidd180147