AKTIVITAS KOMBINASI EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) DAN DAUN PEGAGAN (Centella asiatica L.Urb) TERHADAP KELARUTAN KALSIUM BATU GINJAL SECARA IN VITRO
Abstract: Soursop (Annona
muricata L.) and gotu kola (Centella asiatica L.Urb) is a plant that can be
used as a laxative medicine kidney stones. This is because of the content of
bioactive compounds including flavonoids, especially the leaf section. The use
of a combination of soursop leaf extract and gotu kola can improve their
effectiveness in order to remove calcium kidney stones. Therefore, this study
aims to determine the activity and to determine dose combination soursop leaf
ethanol extract (SS) and gotu kola leaf (GK) which is effective for dissolving
potassium kidney stones. Extracts prepared by maceration method using ethanol
96% then subsequently tested phytochemical screening and thin layer
chromatography profiles on each extract. Results chromatogram showed the
extract containing flavonoids. Testing the activity of the combination of
soursop and gotu kola extract performed using seven treatments, combination 1
(SS 1% + GK 7.5% ), a combination of 2 (SS
0.5% + GK 3.75%), the combination of 3 (SS 0, 25% + GK 1.875%), single
soursop leaf extract (1%), single gotu kola extract (7.5%), negative control
(aquadest) and positive control (Batugin elixir). Kidney stones are then put as
much as 100 mg in each treatment and incubated for 6 hours (37 ℃). Results filtrate then didestruksi and dissolved calcium levels
measured using Atomic Absorption Spectrophotometer. The results showed the
combination soursop leaf ethanol extract and gotu kola leaf extract with
a combination of 2 (SS 0.5% + GK 3.75%) is an effective combination of the
ability to dissolve calcium kidney stones in vitro.
Keywords: Annona muricata
L.;Centella asiatica L.Urb.; Kidney Stones; Calcium
Penulis: Ni Wayan Swintari, Yuliet,
Khilda Khaerati
Kode Jurnal: jpfarmasidd170557
