Cytotoxic Assay From Stem Bark Aglaia minahassae and Aglaia simplicifolia Against HeLa Cervical Cancer Cell Lines
Abstrak: Cervical cancer ranks
as the 2nd leading cause of female cancer in Indonesia. One of healing methods
is chemotherapy, but this method still has many side e ects and also expensive
treatment. Therefore, natural products discoveries need to be developed due to
its important role as an alternative for anti- cancer drug. The aim of this
research was to get IC50 value from methanol, n-hexane, ethyl acetate, and
n-buthanol from stem bark of A. minahassae dan A. simplicifolia. Stem bark of
A. minahassae (1.6 kg) and A. simplicifolia (1.1 kg) was grounded by methanol
and its extract is successively extracted by n-hexane, ethyl acetate, and
n-buthanol. Their extract’s cytotoxicity was then evaluated against HeLa cell
lines. This research showed that A. minahassae’s most cytotoxic extract against
HeLa cell lines was n-hexane (IC50 = 27.4190 μg/mL) and n-buthanol (IC50 =
4.3924 μg/mL). Meanwhile, A. sim- plicifolia most cytotoxic extract extract
against HeLa cell lines was n-hexane (IC50 = 23.3098 μg/mL).
Key words: A. minahassae, A.
simplicifolia, cytotoxic assay, HeLa cell lines
Penulis: Nunung Kurniasih,
Hersa Milawati, Mohamad Fajar, Rizky Abdulah, Desi Harneti Putri Huspa, Unang
Supratman
Kode Jurnal: jpfarmasidd180227
