Cytotoxicity and Antimicrobial Activity of Propolis from Trigona itama Stingless Bees against Staphylococcus aureus and Escherichia coli
Abstrak: Trigona itama is a
stingless bees that produce honey which has been used widely as a traditional
medicine. Due to limited research and knowledge on T. itama propolis, it
becomes less popular in industrial production than honeybee propolis. The aim
of this study was to identify the antibacterial activity and toxicity level in
hexane, ethyl acetate and methanol extracts of T.itama propolis. T.itama
propolis crude extract was tested for antibacterial activity by using disk di
usion method. The antibacterial activities were assessed according to the
inhibitory zone of agar medium with sample concentration of 1000 μg/mL, 750
μg/mL and 500 μg/mL. The result from this study revealed that all T. itama
propolis crude extract shows presence of inhibition zone against Staphylococcus
aureus ATCC 25923 and Escherichia coli ATCC 25922. The brine shrimp lethality
test was used to monitor the toxicity level of T. itama propolis where
methanol, ethyl acetate and hexane fraction showed low activity of toxicity
where all crude extract LC50 showed above 250 μg/mL. It could be concluded from
this study that extract from T. itama propolis possessed antimicrobial activity
and low level of toxicity.
Key Words: Escherichia coli,
propolis, stingless bees, Staphylococcus aureus, Trigona itama
Penulis: Syed A. T. W. Yusop,
Mohd R. Asaruddin, Ahmad H. Sukairi, Wan M. A. W. Sabri
Kode Jurnal: jpfarmasidd180229
